The proliferation of cells represents a fundamental parameter of their functionality. For instance, a high growth rate is frequently associated with a more malignant phenotype whereas arrested cell growth can be the result of immunological defense mechanisms and/or cytotoxic effects of pharmacological agents. Generally, in-vitro toxicity studies at early stages of drug development play a key role in identifying potentially effective compounds.
CellTrend offers two different in-vitro assays for the determination of cellular proliferation:

Luciferase Lucigenin Reaction
Based on bioluminescence detection of intracellular ATP using luciferase lucigenin reaction, our test kit provides a rapid and sensitive (detection limit < 50 cells) assay for high-throughput screening applications.

Colony Formation
For the determination of colonies formed in culture dishes, we utilise bone marrow stem cells and an assortment of tumour cell lines of interest.

Up to date, we have validated our proliferation assays on:

Primary Human Cell Cultures: Endothelial cells Bone marrow cells Leukocytes (subpopulations on request) Smooth muscle cells of the colon Bronchial epithelial cells Mamma epithelial cells Keratinocytes Fibroblastes Human Tumor Cell Lines: Melanoma Colon Cancer Breast cancer Lung carcinoma Leukemia